Making Diluted Solutions of MicroSpheres: Difference between revisions
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* Remove 100ul of cloudy white microsphere solution and dilute with 20ml of deionized water (200:1 dilution). | * Remove 100ul of cloudy white microsphere solution and dilute with 20ml of deionized water (200:1 dilution). | ||
=== Anti-Stick Agents === | |||
* For biological particles BSA and Phosphate-Buffered Saline (PBS - 0.8% NaCl, 0.02% KCL, 0.02M PO4, pH 7.3 (percentage by weight)) can be used as anti-stick agents. | * Bovine Serum Albumin (BSA) at 20mg/ml and (for non-biological particles) 1% polyethylene glycol can be added as anti-stick agents. | ||
* For biological particles the BSA solution and Phosphate-Buffered Saline (PBS - 0.8% NaCl, 0.02% KCL, 0.02M PO4, pH 7.3 (percentage by weight)) can be used as anti-stick agents. | |||
[http://en.wikipedia.org/wiki/Phosphate_buffered_saline PBS Wikipedia] | [http://en.wikipedia.org/wiki/Phosphate_buffered_saline PBS Wikipedia] | ||
''Students: If this recipe is inadequate (too many or too few particles) please revise. '' | ''Students: If this recipe is inadequate (too many or too few particles) please revise. '' | ||
Revision as of 23:07, 24 November 2014
Procedure - From liquid with ~ 10% solids
- Shake microsphere bottle to evenly disperse the particles.
- Remove 100ul of cloudy white microsphere solution and dilute with 20ml of deionized water (200:1 dilution).
Anti-Stick Agents
- Bovine Serum Albumin (BSA) at 20mg/ml and (for non-biological particles) 1% polyethylene glycol can be added as anti-stick agents.
- For biological particles the BSA solution and Phosphate-Buffered Saline (PBS - 0.8% NaCl, 0.02% KCL, 0.02M PO4, pH 7.3 (percentage by weight)) can be used as anti-stick agents.
Students: If this recipe is inadequate (too many or too few particles) please revise.