Optical Tweezers for Biology - Revision history

Aplstudent at 21:26, 16 March 2016

2016-03-16T21:26:08Z

← Older revision		Revision as of 21:26, 16 March 2016
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	-Wear safety glasses at all times while working with the fiber		-Wear safety glasses at all times while working with the fiber
	-The two part polymer should be refrigerated		-The two part polymer should be refrigerated
			-If the fiber head were to be broken into PC head, the whole thing must be thrown away.

Aplstudent at 21:11, 16 March 2016

2016-03-16T21:11:26Z

← Older revision		Revision as of 21:11, 16 March 2016
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	The first thing to try to capture are glass beads. The glass beads are good practice as they have a clear path and direction that they are traveling in. They don't have a tail and will therefore move in a easily predictable path.		The first thing to try to capture are glass beads. The glass beads are good practice as they have a clear path and direction that they are traveling in. They don't have a tail and will therefore move in a easily predictable path.
	There are two types of beads to pick from. There is a vial that had PEG and one that does not contain PEG. PEG is a short name for Polyethylene glycol. It is meant to prevent the beads from being trapped against the glass surface of the coverslip or the slide. The PEG is better for starting to practice and capture the beads. The ones without the PEG is great for fine tuning the apparatus to focus in more on the beads.		There are two types of beads to pick from. There is a vial that had PEG and one that does not contain PEG. PEG is a short name for Polyethylene glycol. It is meant to prevent the beads from being trapped against the glass surface of the coverslip or the slide. The PEG is better for starting to practice and capture the beads. The ones without the PEG is great for fine tuning the apparatus to focus in more on the beads.

	Note:		Note:
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	PEG must be stored in the fridge, otherwise bacteria will form on the beads. This will destroy the beads and make them unusable.		PEG must be stored in the fridge, otherwise bacteria will form on the beads. This will destroy the beads and make them unusable.

	Once you have become good enough at capturing beads, the next step is to catch biological organism. ~~This follows~~ the same ~~process~~ as the beads ~~with the preparation of the slide and the~~		Once you have become good enough at capturing beads, the next step is to catch biological organism. The slide set up for the biological substances should follow the same procedure as the glass beads.


			'''WHAT IS IN THE WATER'''

			There are many microorganisms in the water. Water holds the most abundant range of species. There are things that are large enough to be seen my the naked eye to the microscopic water life. Microscopic creatures

	'''APPLIES PHYSICS'''		'''APPLIES PHYSICS'''

Aplstudent at 20:59, 16 March 2016

2016-03-16T20:59:04Z

← Older revision		Revision as of 20:59, 16 March 2016
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	'''~~STOKES~~ DRAG FORCE'''		'''STOKE'S LAW (DRAG FORCE)'''

Aplstudent at 20:55, 16 March 2016

2016-03-16T20:55:30Z

← Older revision		Revision as of 20:55, 16 March 2016
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	In progress		In progress


			'''STOKES DRAG FORCE'''


			'''LASER CALIBRATION'''


			'''OSCILLATION DEVICE'''

Aplstudent at 20:53, 16 March 2016

2016-03-16T20:53:59Z

← Older revision		Revision as of 20:53, 16 March 2016
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	Once you have become good enough at capturing beads, the next step is to catch biological organism. This follows the same process as the beads with the preparation of the slide and the		Once you have become good enough at capturing beads, the next step is to catch biological organism. This follows the same process as the beads with the preparation of the slide and the


			'''APPLIES PHYSICS'''


			In progress

Aplstudent at 20:48, 16 March 2016

2016-03-16T20:48:20Z

← Older revision		Revision as of 20:48, 16 March 2016
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	[[File:alexpeter15.jpg]]		[[File:alexpeter15.jpg]] [[File:alexpeter16.jpg]]

Aplstudent at 20:46, 16 March 2016

2016-03-16T20:46:03Z

← Older revision		Revision as of 20:46, 16 March 2016
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	'''MICROPIPET ETIQUETTE'''		'''MICROPIPET ETIQUETTE'''


	[[File:alexpeter15.jpg]]		[[File:alexpeter15.jpg]]


	There are a few things that should be known about the micropipet before using it.		There are a few things that should be known about the micropipet before using it.

Aplstudent at 20:44, 16 March 2016

2016-03-16T20:44:38Z

← Older revision		Revision as of 20:44, 16 March 2016
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	'''MICROPIPET ETIQUETTE'''		'''MICROPIPET ETIQUETTE'''
			[[File:alexpeter15.jpg]]

	There are a few things that should be known about the micropipet before using it.		There are a few things that should be known about the micropipet before using it.

Aplstudent at 20:41, 16 March 2016

2016-03-16T20:41:38Z

@@ Line 58: / Line 58: @@
 For the Winter, another addition was added to the apparatus. That was an machine that would oscillate the mount slowly in sin function in order to produce a small movement of the beads. This method was to be used to measure the drag force produced. Since the beads don't move. This is a excellent option to take to measure their movements.
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 . Using nail polish seal off the ends of the coverslip so that the solution won't be able to escape. This will make a close system.
 NOTE:
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 -Gloves must be work to prevent the oils from the hands to be transferred to the coverslip and slide.
 -A new tip for the pipet should be used every time to prevent cross contamination.
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 -The methyl cellulose should be stored in the fridge. If put out for too long it could denature.

Aplstudent at 20:21, 16 March 2016

2016-03-16T20:21:08Z

← Older revision		Revision as of 20:21, 16 March 2016
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	There is a solution known as methyl cellulose. It has the ability to hinder and slow the movement of any microorganism as well as other microscopic mass present. This is the way that we bypassed the problem of the microscopic masses moving pass our field of view. They move extremely quickly and any twitch to focus or lock on to the target would shake it around making it even harder to capture.		There is a solution known as methyl cellulose. It has the ability to hinder and slow the movement of any microorganism as well as other microscopic mass present. This is the way that we bypassed the problem of the microscopic masses moving pass our field of view. They move extremely quickly and any twitch to focus or lock on to the target would shake it around making it even harder to capture.

	~~NOTE~~:		Note:

	-The methyl cellulose should be stored in the fridge. If put out for too long it could denature.		-The methyl cellulose should be stored in the fridge. If put out for too long it could denature.