Optical Tweezers - Revision history

Bsboggs: /* Optical Tweezers */

2018-05-07T19:45:51Z

Optical Tweezers

← Older revision		Revision as of 19:45, 7 May 2018
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	First remove the slide ~~so that you have only~~ the objective, then position a pair of pinholes straight above the objective, and adjust the ~~objective and~~ mirrors until the laser can shoot through both pinholes. That's the rough calibration.		First remove the slide and the objective, then position a pair of (0.5 - 0.75mm) pinholes straight above the objective, and adjust the mirrors until the laser can shoot through both pinholes. That's the rough calibration.
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	Next, put the slide on ~~and~~ and adjust the ~~focus~~ until you can see the interference patterns the laser makes on the slide. Then adjust the ~~mirrors~~ until the interference patterns ~~are~~ are round. Then the laser should be pointed straight at the slide.		Next, put the slide and objective back on and adjust the z-axis until you can see the interference patterns the laser makes on the slide. Then adjust (rotate) the objective until the interference patterns are round. Then the laser should be pointed straight at the slide.
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2016-10-27T17:36:13Z

← Older revision		Revision as of 17:36, 27 October 2016
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	Summer 2016 Powerpoint[http://hank.uoregon.edu/wiki/~~index.php~~/~~File:~~Optical_Tweezers_2016.pdf]		Summer 2016 Powerpoint[http://hank.uoregon.edu/wiki/images/a/a0/Optical_Tweezers_2016.pdf]
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2016-10-27T17:34:59Z

← Older revision		Revision as of 17:34, 27 October 2016
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	<h3>Vortex Wave Retarder</h3>		<h3>Vortex Wave Retarder</h3>
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			Summer 2016 Powerpoint[http://hank.uoregon.edu/wiki/index.php/File:Optical_Tweezers_2016.pdf]
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2016-10-27T16:58:12Z

← Older revision		Revision as of 16:58, 27 October 2016
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	<li>[[Asymmetric Trap: Correction Attempts and Existing Questions]]</li>		<li>[[Asymmetric Trap: Correction Attempts and Existing Questions]]</li>
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	<h3>Vortex Wave Retarder</h3>		<h3>Vortex Wave Retarder</h3>
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2016-10-27T16:43:26Z

← Older revision		Revision as of 16:43, 27 October 2016
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	<li>[[Asymmetric Trap: Correction Attempts and Existing Questions]]</li>		<li>[[Asymmetric Trap: Correction Attempts and Existing Questions]]</li>
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	<h3>Vortex Wave Retarder</h3>		<h3>Vortex Wave Retarder</h3>
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2016-10-27T16:34:16Z

2015-09-05T06:07:45Z

← Older revision		Revision as of 06:07, 5 September 2015
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	<h3>Our own setup</h3>		<h3>Our own setup</h3>
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	<li>		<li> '''Objective lens alignment & care'''
			*To align the objective lens so that it is mounted parallel to the optical bench surface, use a level on the objective mount.

			[[File:obj 1.JPG\|400px]]
			*Make an oil guard to keep immersion oil off the main chassis of the objective by cutting a small hole in the center of a square of Parafilm and pushing the objective through.

			[[File:obj 2.JPG\|400px]]
			*To remove oil from the objective lens, carefully use a lens tissue around the end of the lens to draw oil off the lens using capillary action without actually touching the delicate output lens.

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	Slide Setup		Slide Setup
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2015-09-05T01:28:38Z

← Older revision		Revision as of 01:28, 5 September 2015
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	The pattern matching does work quite well for the purposes of tracking brownian motion. You need to create a pattern matching script and then run it through batch processing.		The pattern matching does work quite well for the purposes of tracking brownian motion. You need to create a pattern matching script and then run it through batch processing.
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			During batch processing, pay attention to the red and green tracking squares as the images are being processed. If these squares do not appear, the program is likely not tracking the object position accurately.
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2015-03-16T22:25:20Z

Optical Tweezers

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 We actually tried both using pattern matching and brute force point and click methods.  Neither worked very well, so we recommend using the QPD to get position measurements.  You don't need an actual video for this.
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2014-12-10T20:32:47Z

Optical Tweezers

← Older revision		Revision as of 20:32, 10 December 2014
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	[http://www.instructables.com/id/Image-Acquisition-in-NI-Vision-Assistant/] Image Acquisition/Saving Images		[http://www.instructables.com/id/Image-Acquisition-in-NI-Vision-Assistant/] Image Acquisition/Saving Images. The UI for this program is rather difficult. To exit from video capture mode after capturing images there is a button that says close near the bottom left, underneath the camera options window.
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	We actually tried both using pattern matching and brute force point and click methods. Neither worked very well, so we recommend using the QPD to get position measurements		To get an actual video, you need to run the pictures through another video editing program. There is one on the computer, but there is no video option in NI Vision Assistant itself.
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			We actually tried both using pattern matching and brute force point and click methods. Neither worked very well, so we recommend using the QPD to get position measurements. You don't need an actual video for this.
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